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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VPS52 Polyclonal antibody (11662-2-AP)

    WB analysis of HepG2 using 11662-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using VPS52 Polyclonal antibody (11662-2-AP)

    WB analysis of mouse skin using 11662-2-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using VPS52 Polyclonal antibody (11662-2-AP)

    WB analysis of COLO 320 using 11662-2-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skin tissue using VPS52 Polyclonal antibody (11662-2-AP)

    IP experiment of mouse skin using 11662-2-AP

    IP result of anti-VPS52 (IP:11662-2-AP, 3ug; Detection:11662-2-AP 1:1000) with mouse skin tissue lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using VPS52 Polyclonal antibody (11662-2-AP)

    IHC staining of human gliomas using 11662-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11662-2-AP (VPS52 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS52 Polyclonal antibody (11662-2-AP)

    IF Staining of HepG2 using 11662-2-AP

    Immunofluorescent analysis of HepG2 cells using 11662-2-AP (VPS52 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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VPS52 Polyclonal antibody

VPS52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11662-2-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Vacuolar protein sorting-associated protein 52 homolog, SACM2L, SAC2 suppressor of actin mutations 2-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, mouse skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11662-2-AP targets VPS52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS52 fusion protein Ag2180 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 52 homolog (S. cerevisiae)
Calculated molecular weight 723 aa, 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC032108
Gene Symbol VPS52
Gene ID (NCBI) 6293
RRIDAB_2241614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1B4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).

Protocols

Product Specific Protocols
WB protocol for VPS52 antibody 11662-2-APDownload protocol
IHC protocol for VPS52 antibody 11662-2-APDownload protocol
IF protocol for VPS52 antibody 11662-2-APDownload protocol
IP protocol for VPS52 antibody 11662-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.

Authors - Jian Wei
View Article
humanIHC, WB

J Mol Med (Berl)

VPS52 induces apoptosis via cathepsin D in gastric cancer.

Authors - Jian Zhang
View Article