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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS53 Polyclonal antibody

VPS53 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12824-1-AP

Synonyms

Vacuolar protein sorting-associated protein 53 homolog, PP13624

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using VPS53 Polyclonal antibody (12824-1-AP)

    WB analysis of mouse liver using 12824-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using VPS53 Polyclonal antibody (12824-1-AP)

    WB analysis of human liver using 12824-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using VPS53 Polyclonal antibody (12824-1-AP)

    WB analysis of L02 using 12824-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using VPS53 Polyclonal antibody (12824-1-AP)

    IP experiment of mouse liver using 12824-1-AP

    IP result of anti-VPS53 (IP:12824-1-AP, 4ug; Detection:12824-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using VPS53 Polyclonal antibody (12824-1-AP)

    IHC staining of human stomach using 12824-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12824-1-AP (VPS53 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12824-1-AP targets VPS53 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS53 fusion protein Ag3518 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Calculated molecular weight 832 aa, 94 kDa
Observed molecular weight 95-97 kDa
GenBank accession numberBC029560
Gene symbol VPS53
Gene ID (NCBI) 55275
RRIDAB_2215514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

Protocols

Product Specific Protocols
WB protocol for VPS53 antibody 12824-1-APDownload protocol
IHC protocol for VPS53 antibody 12824-1-APDownload protocol
IP protocol for VPS53 antibody 12824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

Biallelic variants in VPS50 cause a neurodevelopmental disorder with neonatal cholestasis.

Authors - Pauline E Schneeberger
View Article