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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS54 Monoclonal antibody, PBS Only

VPS54 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D12D1

Cat no : 67404-1-PBS

Synonyms

HCC8, hVps54L, SLP 8p, Tumor antigen HOM HCC 8, Tumor antigen SLP 8p, VPS54, VPS54L

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using VPS54 Monoclonal antibody (67404-1-Ig)

    WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using VPS54 Monoclonal antibody (67404-1-Ig)

    WB analysis of pig brain using 67404-1-Ig (same clone as 67404-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS54 Monoclonal antibody (67404-1-Ig)

    IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS54 Monoclonal antibody (67404-1-Ig)

    IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67404-1-PBS targets VPS54 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS54 fusion protein Ag4123 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 54 homolog (S. cerevisiae)
Calculated molecular weight 977 aa, 111 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC030275
Gene symbol VPS54
Gene ID (NCBI) 51542
RRIDAB_2882645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.