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  • Featured Product
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VPS72 Polyclonal antibody

VPS72 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15143-1-AP

Synonyms

CFL1, Protein YL 1, Swc2, TCFL1, Transcription factor like 1, VPS72, YL 1, YL1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using VPS72 Polyclonal antibody (15143-1-AP)

    WB analysis of HeLa using 15143-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using VPS72 Polyclonal antibody (15143-1-AP)

    WB analysis of A549 using 15143-1-AP

    WB result of VPS72 antibody (15143-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS72 transfected A549 cells.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using VPS72 Polyclonal antibody (15143-1-AP)

    WB analysis of mouse skeletal muscle using 15143-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using VPS72 Polyclonal antibody (15143-1-AP)

    WB analysis of NIH/3T3 using 15143-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using VPS72 Polyclonal antibody (15143-1-AP)

    IP experiment of NIH/3T3 using 15143-1-AP

    IP result of anti-VPS72 (IP:15143-1-AP, 4ug; Detection:15143-1-AP 1:500) with NIH/3T3 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using VPS72 Polyclonal antibody (15143-1-AP)

    IHC staining of human breast hyperplasia using 15143-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using VPS72 Polyclonal antibody (15143-1-AP)

    IHC staining of human breast hyperplasia using 15143-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using VPS72 Polyclonal antibody (15143-1-AP)

    IHC staining of human breast hyperplasia using 15143-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using VPS72 Polyclonal antibody (15143-1-AP)

    IHC staining of human breast hyperplasia using 15143-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, NIH/3T3 cells, A549 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15143-1-AP targets VPS72 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS72 fusion protein Ag7424 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 72 homolog (S. cerevisiae)
Calculated molecular weight 41 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC003151
Gene symbol VPS72
Gene ID (NCBI) 6944
RRIDAB_2215534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex, which exists in Drosophila melanogaster and mammalian cells, is a complex that is responsible for various cellular processes, including DNA repair, transcriptional activation and apoptosis. Vacuolar protein sorting-associated protein 72 homolog (VPS72), also known as VPS72 or Transcription factor-like 1, is a 364 amino acid subunit of the TRRAP/TIP60 HAT complex. VPS72 has also been identified as a subunit of a novel complex containing SNF2-related helicase SRCAP (SWI2/SNF2-related CBP activator protein). This SRCAP-containing complex is very similar to the S. cerevisiae SWR1 chromatin remodeling complex. The involvement of VPS72 in these complexes has suggested that VPS72 plays multiple roles in chromatin modification and remodeling in cells. VPS72 localizes to the nucleus and is phosphorylated upon DNA damage, most likely by ATM or ATR.

Protocols

Product Specific Protocols
WB protocol for VPS72 antibody 15143-1-APDownload protocol
IHC protocol for VPS72 antibody 15143-1-APDownload protocol
IP protocol for VPS72 antibody 15143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

PMID: 28366643

Authors - Sophie Barral
View Article
WB

Cell Discov

Structural insights into histone exchange by human SRCAP complex

Authors - Jiali Yu
View Article