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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VSIG1 Polyclonal antibody

VSIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14145-1-AP

Synonyms

V-set and immunoglobulin domain-containing protein 1, GPA34, Glycoprotein A34, Cell surface A33 antigen

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human stomach tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    WB analysis of human stomach using 14145-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14145-1-AP (VSIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    WB analysis of human stomach using 14145-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14145-1-AP (VSIG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    IHC staining of human testis using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14145-1-AP (VSIG1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    IHC staining of human testis using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14145-1-AP (VSIG1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    IHC staining of human stomach using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14145-1-AP (VSIG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    IHC staining of human testis using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14145-1-AP (VSIG1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using VSIG1 Polyclonal antibody (14145-1-AP)

    IHC staining of human stomach using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14145-1-AP (VSIG1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman stomach tissue
Positive IHC detected inhuman stomach tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14145-1-AP targets VSIG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSIG1 fusion protein Ag5314 相同性解析による交差性が予測される生物種
Full Name V-set and immunoglobulin domain containing 1
Calculated molecular weight 387 aa, 42 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC043216
Gene symbol VSIG1
Gene ID (NCBI) 340547
RRIDAB_2272980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VSIG1 antibody 14145-1-APDownload protocol
IHC protocol for VSIG1 antibody 14145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

Downregulation of cancer-associated fibroblast exosome-derived miR-29b-1-5p restrains vasculogenic mimicry and apoptosis while accelerating migration and invasion of gastric cancer cells via immunoglobulin domain-containing 1/zonula occluden-1 axis

Authors - Chenqu Wu
View Article