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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VILIP-1 Polyclonal antibody

VILIP-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13919-1-AP

Synonyms

VILIP 1, VILIP1, VSNL1, VILIP, HUVISL1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VILIP-1 Polyclonal antibody (13919-1-AP)

    WB analysis using 13919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse tongue tissue using VILIP-1 Polyclonal antibody (13919-1-AP)

    WB analysis of mouse tongue using 13919-1-AP

    mouse tongue tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using VILIP-1 Polyclonal antibody (13919-1-AP)

    WB analysis of human brain using 13919-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using VILIP-1 Polyclonal antibody (13919-1-AP)

    WB analysis of rat brain using 13919-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VILIP-1 Polyclonal antibody (13919-1-AP)

    WB analysis of HeLa using 13919-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VILIP-1 Polyclonal antibody (13919-1-AP)

    IP experiment of mouse brain using 13919-1-AP

    IP result of anti-VILIP-1 (IP:13919-1-AP, 3ug; Detection:13919-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • Immunohistochemistry (IHC) staining of Human bowens disease using VILIP-1 Polyclonal antibody (13919-1-AP)

    IHC staining of Human bowens disease using 13919-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13919-1-AP (VILIP-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VILIP-1 Polyclonal antibody (13919-1-AP)

    IF Staining of HeLa using 13919-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VILIP-1 antibody (13919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse tongue tissue, human brain tissue, rat brain tissue, HeLa cells, mouse cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inHuman bowens disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13919-1-AP targets VILIP-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VILIP-1 fusion protein Ag4923 相同性解析による交差性が予測される生物種
Full Name visinin-like 1
Calculated molecular weight 22 kDa
Observed molecular weight 22-24 kDa
GenBank accession numberBC022012
Gene symbol VSNL1
Gene ID (NCBI) 7447
RRIDAB_2215851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VILIP-1 antibody 13919-1-APDownload protocol
IHC protocol for VILIP-1 antibody 13919-1-APDownload protocol
IF protocol for VILIP-1 antibody 13919-1-APDownload protocol
IP protocol for VILIP-1 antibody 13919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB,IF

Free Radic Biol Med

Dimerization of visinin-like-protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis.

Authors - Martina P Liebl
View Article
mouseWB

Front Cell Neurosci

Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.

Authors - Anna Sancho-Balsells
View Article