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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VSTM1 Polyclonal antibody

VSTM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24382-1-AP

Synonyms

UNQ3033, VSTM1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using VSTM1 Polyclonal antibody (24382-1-AP)

    WB analysis using 24382-1-AP

    human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 24382-1-AP (VSTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using VSTM1 Polyclonal antibody (24382-1-AP)

    WB analysis of HL-60 using 24382-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 24382-1-AP (VSTM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VSTM1 Polyclonal antibody (24382-1-AP)

    IHC staining of human tonsillitis using 24382-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24382-1-AP (VSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VSTM1 Polyclonal antibody (24382-1-AP)

    IHC staining of human tonsillitis using 24382-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24382-1-AP (VSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman peripheral blood leukocyte, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24382-1-AP is VSTM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSTM1 fusion protein Ag15799 相同性解析による交差性が予測される生物種
Full Name V-set and transmembrane domain containing 1
Calculated molecular weight 236 aa, 26 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC100942
Gene symbol VSTM1
Gene ID (NCBI) 284415
RRIDAB_2879516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VSTM1 (V-set and transmembrane domain-containing protein 1) has two main splicing isoform, VSTM1-v1 and VSTM1-v2. VSTM1-v1 is a type I membrane molecule, mainly expressed on human peripheral blood granulocytes and monocytes. VSTM1-v2 is a classical secretory glycoprotein mainly expressed in immune tissues, and can promote the differentiation and activation of Th17 cells. (PMID: 22960280; 23909423)

Protocols

Product Specific Protocols
WB protocol for VSTM1 antibody 24382-1-APDownload protocol
IHC protocol for VSTM1 antibody 24382-1-APDownload protocol
FC protocol for VSTM1 antibody 24382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Mol Immunol

Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils

Authors - Xu Jiang
View Article