VSTM1 Polyclonal antibody
VSTM1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24382-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human peripheral blood leukocyte, HL-60 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IP | See 1 publications below |
Product Information
The immunogen of 24382-1-AP is VSTM1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VSTM1 fusion protein Ag15799 相同性解析による交差性が予測される生物種 |
Full Name | V-set and transmembrane domain containing 1 |
Calculated molecular weight | 236 aa, 26 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC100942 |
Gene symbol | VSTM1 |
Gene ID (NCBI) | 284415 |
RRID | AB_2879516 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VSTM1 (V-set and transmembrane domain-containing protein 1) has two main splicing isoform, VSTM1-v1 and VSTM1-v2. VSTM1-v1 is a type I membrane molecule, mainly expressed on human peripheral blood granulocytes and monocytes. VSTM1-v2 is a classical secretory glycoprotein mainly expressed in immune tissues, and can promote the differentiation and activation of Th17 cells. (PMID: 22960280; 23909423)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for VSTM1 antibody 24382-1-AP | Download protocol |
IHC protocol for VSTM1 antibody 24382-1-AP | Download protocol |
FC protocol for VSTM1 antibody 24382-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Mol Immunol Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils |