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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using VSX1 Polyclonal antibody (23566-1-AP)

    WB analysis of mouse eye using 23566-1-AP (same clone as 23566-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using VSX1 Polyclonal antibody (23566-1-AP)

    WB analysis of Raji using 23566-1-AP (same clone as 23566-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using VSX1 Polyclonal antibody (23566-1-AP)

    IF Staining of mouse eye using 23566-1-AP (same clone as 23566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using 23566-1-AP (VSX1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23566-1-PBS in a different storage buffer formulation.

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VSX1 Polyclonal antibody, PBS Only

VSX1 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 23566-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23566-1-PBS targets VSX1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSX1 fusion protein Ag19222 相同性解析による交差性が予測される生物種
Full Name visual system homeobox 1
Calculated molecular weight 365 aa, 38 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC126228
Gene Symbol VSX1
Gene ID (NCBI) 30813
RRIDAB_2879297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZR4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VSX1(visual system homeobox 1) is a member of the Vsx1 group of vertebrate paired-like homeodomain transcription factors locating to the nucleus. Mutations in the VSX1 gene in keratoconus have been reported in many studies. VSX1 is considered important in ocular development and is particularly involved in the developing cornea (PMID:21139977). And previous studies have identified that Vsx1 participates in regulating retinal progenitor proliferation, differentiation and functional maintenance of bipolar cells (PMID:25150888). It was demonstrated that Vsx1 express in embryonic craniofacial, adult corneal, and adult retinal cDNA libraries.VSX1 mRNA has been found in the outer tier of the inner nuclear layer of the human retina and the cornea (PMID: 21139977).