VSX2 Polyclonal antibody
VSX2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 25825-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse eye tissue |
Positive IHC detected in | human retinoblastoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse eye tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 2 publications below |
Product Information
25825-1-AP targets VSX2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VSX2 fusion protein Ag23035 相同性解析による交差性が予測される生物種 |
Full Name | visual system homeobox 2 |
Calculated molecular weight | 361 aa, 39 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC128153 |
Gene symbol | VSX2 |
Gene ID (NCBI) | 338917 |
RRID | AB_2880257 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for VSX2 antibody 25825-1-AP | Download protocol |
IHC protocol for VSX2 antibody 25825-1-AP | Download protocol |
IF protocol for VSX2 antibody 25825-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Stem Cell Human brain organoids assemble functionally integrated bilateral optic vesicles. | ||
Nat Protoc Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alessandro (Verified Customer) (11-06-2022) | no unspecific staining, great outcome
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