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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VSX2 Polyclonal antibody

VSX2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25825-1-AP

Synonyms

MCOPCB3, MCOP2, HOX10, Homeobox protein CHX10, CHX10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse eye tissue using VSX2 Polyclonal antibody (25825-1-AP)

    WB analysis of mouse eye using 25825-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 25825-1-AP (VSX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human retinoblastoma tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human retinoblastoma tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human lung cancer using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human lung cancer using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IF Staining of mouse eye using 25825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman retinoblastoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

25825-1-AP targets VSX2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSX2 fusion protein Ag23035 相同性解析による交差性が予測される生物種
Full Name visual system homeobox 2
Calculated molecular weight 361 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC128153
Gene symbol VSX2
Gene ID (NCBI) 338917
RRIDAB_2880257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VSX2 antibody 25825-1-APDownload protocol
IHC protocol for VSX2 antibody 25825-1-APDownload protocol
IF protocol for VSX2 antibody 25825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
View Article
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

  • Applications: Immunofluorescence