Vitronectin Polyclonal antibody
Vitronectin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 15833-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat plasma, human plasma, human testis tissue, NIH/3T3 cells |
| Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
Product Information
15833-1-AP targets Vitronectin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, pig, plasmodium falciparum |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Vitronectin fusion protein Ag8443 相同性解析による交差性が予測される生物種 |
| Full Name | vitronectin |
| Calculated molecular weight | 478 aa, 54 kDa |
| Observed molecular weight | 70-75 kDa, 65 kDa, |
| GenBank accession number | BC005046 |
| Gene symbol | VTN |
| Gene ID (NCBI) | 7448 |
| RRID | AB_2216309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Vitronectin antibody 15833-1-AP | Download protocol |
| IHC protocol for Vitronectin antibody 15833-1-AP | Download protocol |
| IF protocol for Vitronectin antibody 15833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury | ||
Brain Pathol Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix. | ||
Sci Rep Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. | ||
J Cell Mol Med Induction of migration of periodontal ligament cells by selective regulation of integrin subunits. | ||
Langmuir Characterization of Initial Cell Adhesion on Charged Polymer Substrates in Serum-Containing and Serum-Free Media. | ||
Proteomics SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jingwen (Verified Customer) (01-28-2020) | It worked well when I performed the western blot in cancer samples.
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