Validation Data Gallery
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Tested Applications
| Positive WB detected in | T-47D cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
14322-1-AP targets VWA1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VWA1 fusion protein Ag5565 相同性解析による交差性が予測される生物種 |
| Full Name | von Willebrand factor A domain containing 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC059409 |
| Gene Symbol | VWA1 |
| Gene ID (NCBI) | 64856 |
| RRID | AB_2241694 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PCB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VWA1 antibody 14322-1-AP | Download protocol |
| IHC protocol for VWA1 antibody 14322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling. |