• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VWA1 Polyclonal antibody

VWA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14322-1-AP

Synonyms

VWA 1, VWA1, WARP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of T-47D cells using VWA1 Polyclonal antibody (14322-1-AP)

    WB analysis of T-47D using 14322-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 14322-1-AP (VWA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VWA1 Polyclonal antibody (14322-1-AP)

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VWA1 Polyclonal antibody (14322-1-AP)

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VWA1 Polyclonal antibody (14322-1-AP)

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14322-1-AP (VWA1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VWA1 Polyclonal antibody (14322-1-AP)

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14322-1-AP (VWA1 antibody) at dilution of 1:50 (under 40x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inT-47D cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14322-1-AP targets VWA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VWA1 fusion protein Ag5565 相同性解析による交差性が予測される生物種
Full Name von Willebrand factor A domain containing 1
Calculated molecular weight 47 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC059409
Gene symbol VWA1
Gene ID (NCBI) 64856
RRIDAB_2241694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier.

Protocols

Product Specific Protocols
WB protocol for VWA1 antibody 14322-1-APDownload protocol
IHC protocol for VWA1 antibody 14322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling.

Authors - Qin Zou
View Article