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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using VWA2 Polyclonal antibody (18714-1-AP)

    WB analysis of A375 using 18714-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18714-1-AP (VWA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon using 18714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon using 18714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon cancer using 18714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 18714-1-AP (VWA2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon cancer using 18714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 18714-1-AP (VWA2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using VWA2 Polyclonal antibody (18714-1-AP)

    IF Staining of A375 using 18714-1-AP

    Immunofluorescent analysis of A375 cells using 18714-1-AP (VWA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VWA2 Polyclonal antibody (18714-1-AP)

    IF Staining of HEK-293 using 18714-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using VWA2 antibody (18714-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

VWA2 Polyclonal antibody

VWA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18714-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

von Willebrand factor A domain-containing protein 2, NET42, CCSP 2, AMACO, A domain-containing protein similar to matrilin and collagen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18714-1-AP targets VWA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name von Willebrand factor A domain containing 2
Calculated molecular weight 82 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC128588
Gene Symbol VWA2
Gene ID (NCBI) 340706
RRIDAB_10598772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5GFL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VWA2, also named as AMACO and CCSP2, is a novel candidate serological marker of colon neoplasia. VWA2 is found in the media of variesy of established cell lines of both fibroblast and epithelial origin. This antibody can recognize all the 3 isoforms of VWA2.

Protocols

Product Specific Protocols
WB protocol for VWA2 antibody 18714-1-APDownload protocol
IHC protocol for VWA2 antibody 18714-1-APDownload protocol
IF protocol for VWA2 antibody 18714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols