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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VWF Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.313-20 ng/mL

Cat No : MP00810-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00810-3, Human VWF Recombinant Matched Antibody Pair - PBS only. 83854-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25578. 83854-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

    Sandwich ELISA standard curve of MP00810-3

    Sandwich ELISA standard curve of MP00810-3, Human VWF Recombinant Matched Antibody Pair - PBS only. 83854-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25578. 83854-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Serum of sixteen individual healthy human donors was measured. The VWF concentration of detected samples was determined to be 239.02 ng/mL with a range of 33.91-609.64 ng/mL.

    Sample test of MP00810-3

    Serum of sixteen individual healthy human donors was measured. The VWF concentration of detected samples was determined to be 239.02 ng/mL with a range of 33.91-609.64 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00810-3 targets VWF in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: vwf Recombinant antibody, PBS Only (Capture) 83854-4-PBS (240867D1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: vwf Recombinant antibody, PBS Only (Detector) 83854-1-PBS (240867D7). 100 μg. Concentration 1 mgl/ml.

Alternative VWF matched antibody pairs: MP00810-1, MP00810-2

Matched pair protein standard: Ag25578

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83854-4-PBS
Tested Reactivity human
Immunogen vwf fusion protein Ag25578
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name von Willebrand factor
Synonyms VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D1
Gene symbol VWF
UniprotP04275
Gene ID (NCBI) 7450
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83854-1-PBS
Tested Reactivity human
Immunogen vwf fusion protein Ag25578
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name von Willebrand factor
Synonyms VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D7
Gene symbol VWF
UniprotP04275
Gene ID (NCBI) 7450
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.