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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ValRS Monoclonal antibody

ValRS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2C3

Cat no : 67935-1-Ig

Synonyms

G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ValRS Monoclonal antibody (67935-1-Ig)

    WB analysis using 67935-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using ValRS Monoclonal antibody (67935-1-Ig)

    WB analysis of LNCaP using 67935-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ValRS Monoclonal antibody (67935-1-Ig)

    WB analysis of MCF-7 using 67935-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ValRS Monoclonal antibody (67935-1-Ig)

    IHC staining of human tonsillitis using 67935-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67935-1-Ig (ValRS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ValRS Monoclonal antibody (67935-1-Ig)

    IF Staining of HeLa using 67935-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ValRS antibody (67935-1-Ig, Clone: 1A2C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, MCF-7 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67935-1-Ig targets ValRS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ValRS fusion protein Ag9312 相同性解析による交差性が予測される生物種
Full Name valyl-tRNA synthetase
Calculated molecular weight 1264 aa, 140 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC012808
Gene symbol ValRS
Gene ID (NCBI) 7407
RRIDAB_2918687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ValRS antibody 67935-1-IgDownload protocol
IHC protocol for ValRS antibody 67935-1-IgDownload protocol
IF protocol for ValRS antibody 67935-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols