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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Vaspin Monoclonal antibody

Vaspin Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1B6

Cat no : 67573-1-Ig

Synonyms

SERPINA12, Visceral adipose tissue-derived serine protease inhibitor, Serpin A12, OL-64, OL 64

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Vaspin Monoclonal antibody (67573-1-Ig)

    WB analysis using 67573-1-Ig

    Human adipose tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Vaspin Monoclonal antibody (67573-1-Ig)

    WB analysis of human placenta using 67573-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Vaspin Monoclonal antibody (67573-1-Ig)

    IF Staining of HeLa using 67573-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vaspin antibody (67573-1-Ig, Clone: 2A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inhuman adipose tissue, human placenta tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67573-1-Ig targets Vaspin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vaspin fusion protein Ag30136 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC040857
Gene symbol Vaspin
Gene ID (NCBI) 145264
RRIDAB_2882786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Vaspin antibody 67573-1-IgDownload protocol
IF protocol for Vaspin antibody 67573-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols