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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VEGFA Recombinant antibody

VEGFA Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5

Cat no : 82864-16-RR

Synonyms

Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mouse Vegfa Recombinant antibody (82864-16-RR)

    WB analysis using 82864-16-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82864-16-RR (Vegfa antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82864-16-RR

    Affinity and Kinetic Characterization of 82864-16-RR

    Biolayer interferometry (BLl) kinetic assays of 82864-16-RR against Mouse VEGFA were performed. The affinity constant is 0.505 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells, MDA-MB-231 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82864-16-RR targets VEGFA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name vascular endothelial growth factor A
Observed molecular weight38 kDa
GenBank accession numberNM_001287056
Gene symbol Vegfa
Gene ID (NCBI) 22339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

Protocols

Product Specific Protocols
WB protocol for VEGFA antibody 82864-16-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols