Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method)
EthD-I (DNA binded): Ex/Em
528/617 nm
Calcein AM：Ex/Em
494/517 nm
Application
FC
Cat no : PF00008
Validation Data Gallery
Product Information
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-I method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems.
This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Components | 150T | 300T |
A.Calcein AM (4 mM in anhydrous DMSO) | 50 uL | 100 uL |
B.Ethidium homodimer-1 (EthD-I) (2 mM in DMSO/H2O 1:4 (v/v)) | 150 uL | 300 uL |
Note: The test size is determined as 0.5 mL working solution for one sample in flow cytometry.
Storage
Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment.
Cautions
Note that Calcein M is easy to hydrolyze, so it needs to be sealed and stored dry, and the diluted working solution needs to be prepared the same day.
1. Fluorescent dyes all have quenching problems. Please try to avoid light during storage and use to slow down fluorescence quenching.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
Spectrum
Calcein AM：Ex/Em = 494/517 nm
EthD-I: Ex/Em = 528/617 nm (binding DNA)
Publications
| Application | Title |
|---|---|
Cell Stem Cell Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids | |
Front Endocrinol (Lausanne) Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM2.5. | |
Sci Total Environ PM2.5 caused ferroptosis in spermatocyte via overloading iron and disrupting redox homeostasis | |
JOR Spine Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis | |
Nat Commun Multiscale architecture design of 3D printed biodegradable Zn-based porous scaffolds for immunomodulatory osteogenesis |