Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)
Cat no : PF00007
Validation Data Gallery
Product Information
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems.
This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Components | 150T | 300T |
A. Calcein AM (4 mM in anhydrous DMSO) | 50 uL | 100 uL |
B. Propidium (PI) (1.5 mM in H2O) | 250 uL | 500 uL |
Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.
Storage
Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment.
Cautions
1. Fluorescent dyes all have quenching problems.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
Parameters
CalceinAM：Ex/Em=494/517nm
PI: Ex/Em=535/617nm (with DNA)
Publications
| Application | Title |
|---|---|
ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma | |
Adv Sci (Weinh) Regulating Type H Vessel Formation and Bone Metabolism via Bone-Targeting Oral Micro/Nano-Hydrogel Microspheres to Prevent Bone Loss | |
Adv Healthc Mater Long-lived Photoacid-doped Conducting Composites Induce Photocurrent for Efficient Wound Healing | |
FASEB J HDM induce airway epithelial cell ferroptosis and promote inflammation by activating ferritinophagy in asthma. | |
J Cardiovasc Transl Res CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes. | |
Drug Des Devel Ther Curcumin- and Cyclopamine-Loaded Liposomes to Enhance Therapeutic Efficacy Against Hepatic Fibrosis. |