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  • Featured Product
  • KD/KO Validated

Vimentin Monoclonal antibody, PBS Only (Detector)

Vimentin Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

3H9D1

Cat no : 60330-1-PBS

Synonyms

VIM, Vimentin

Validation Data Gallery

Applications:
  • WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

    WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis of NIH/3T3 using 60330-1-Ig (same clone as 60330-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • WB analysis of ROS1728 using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis of ROS1728 using 60330-1-Ig (same clone as 60330-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human colon using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:102400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human breast cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human liver cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human ovary tumor using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human tonsillitis using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human pancreas using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human pancreas using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human renal cell carcinoma using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig (Vimentin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human renal cell carcinoma using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig (Vimentin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human endometrial cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60330-1-Ig (same clone as 60330-1-PBS)

    IF Staining of HepG2 using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

    IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IF Staining of Retinal organoids using 60330-1-Ig (same clone as 60330-1-PBS)

    IF Staining of Retinal organoids using 60330-1-Ig (same clone as 60330-1-PBS)

    Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Vimentin with 60330-1-Ig at 1:500 dilution (green) and Caspase 3 using 19677-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 60330-1-Ig (same clone as 60330-1-PBS)

    FC experiment of Jurkat using 60330-1-Ig (same clone as 60330-1-PBS)

    1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human Vimentin (60330-1-Ig, Clone:3H9D1) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50158-1

    Cytometric bead array standard curve of MP50158-1

    Cytometric bead array standard curve of MP50158-1, Vimentin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60330-2-PBS. Detection antibody: 60330-1-PBS. Standard:ag0489. Range: 3.125-100 ng/mL.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60330-1-PBS targets Vimentin as part of a matched antibody pair:

MP50158-1: 60330-2-PBS capture and 60330-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.