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  • Featured Product
  • KD/KO Validated

Vimentin Recombinant antibody, PBS Only

Vimentin Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6K21

Cat no : 80232-1-PBS

Synonyms

VIM, 6K21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Vimentin Recombinant antibody (80232-1-RR)

    WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Vimentin Recombinant antibody (80232-1-RR)

    WB analysis of A549 using 80232-1-RR (same clone as 80232-1-PBS)

    WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Vimentin Recombinant antibody (80232-1-RR)

    WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vimentin Recombinant antibody (80232-1-RR)

    IHC staining of human breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Vimentin Recombinant antibody (80232-1-RR)

    IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Vimentin Recombinant antibody (80232-1-RR)

    IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Vimentin Recombinant antibody (80232-1-RR)

    IHC staining of human liver cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vimentin Recombinant antibody (80232-1-RR)

    IF Staining of HepG2 using 80232-1-RR (same clone as 80232-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80232-1-PBS targets Vimentin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).