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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat no : 66305-1-PBS

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis of pig heart using 66305-1-Ig (same clone as 66305-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of mouse heart using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of mouse heart using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human prostate cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human prostate cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Vinculin Monoclonal antibody (66305-1-Ig)

    IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66305-1-PBS targets Vinculin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946 相同性解析による交差性が予測される生物種
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol Vinculin
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.