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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Vinculin Monoclonal antibody (66305-2-Ig)

    IHC staining of human ovary cancer using 66305-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Vinculin Monoclonal antibody (66305-2-Ig)

    IHC staining of human ovary cancer using 66305-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Vinculin Monoclonal antibody (66305-2-Ig)

    IF Staining of hTERT-RPE1 using 66305-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66305-2-Ig
Clone No.3A11F8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHGC-27 cells, HUVEC cells, LNCaP cells, MKN-45 cells, K-562 cells, human peripheral blood platelets, rat heart tissue, mouse heart tissue, rabbit heart tissue, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66305-2-Ig targets Vinculin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946 相同性解析による交差性が予測される生物種
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight124 kDa
GenBank accession numberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_3670375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 66305-2-IgDownload protocol
IHC protocol for Vinculin antibody 66305-2-IgDownload protocol
IF protocol for Vinculin antibody 66305-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols