CoraLite® Plus 750-conjugated Vinculin Monoclonal antibody
Vinculin Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2B5A7
Cat no : CL750-66305
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-66305 targets Vinculin in WB applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Vinculin fusion protein Ag24946 相同性解析による交差性が予測される生物種 |
Full Name | vinculin |
Calculated molecular weight | 1133 aa, 124 kDa |
Observed molecular weight | 117 kDa |
GenBank accession number | BC039174 |
Gene symbol | Vinculin |
Gene ID (NCBI) | 7414 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CL Plus 750 Vinculin antibody CL750-66305 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |