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  • KD/KO Validated

WAPL Polyclonal antibody

WAPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16370-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

WAPL/FOE, FOE, Wings apart-like protein homolog, WAPL cohesin release factor, WAPAL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16370-1-AP targets WAPL in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WAPL fusion protein Ag9280 相同性解析による交差性が予測される生物種
Full Name wings apart-like homolog (Drosophila)
Calculated molecular weight 142 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberBC017393
Gene Symbol WAPL
Gene ID (NCBI) 23063
RRIDAB_2216716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5K2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

Protocols

Product Specific Protocols
WB protocol for WAPL antibody 16370-1-APDownload protocol
IHC protocol for WAPL antibody 16370-1-APDownload protocol
IF protocol for WAPL antibody 16370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,ChIP

Nat Genet

WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation.

Authors - Ning Qing Liu
  • KO Validated
humanWB

Sci Adv

RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis.

Authors - Shu Zhang
mouseWB

J Clin Invest

Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.

Authors - Sun Yaping Y
mouseWB

Genome Biol

CTCF and cohesin promote focal detachment of DNA from the nuclear lamina

Authors - Tom van Schaik
humanWB

Curr Biol

Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2.

Authors - Masashi Minamino
mouseWB,IF

Cell Rep

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.

Authors - Miguel A Brieño-Enríquez
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