Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67620-1-Ig targets WASF3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | WASF3 fusion protein Ag29991 相同性解析による交差性が予測される生物種 |
| Full Name | WAS protein family, member 3 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 50-55 and 66-70 kDa |
| GenBank accession number | BC050283 |
| Gene Symbol | WASF3 |
| Gene ID (NCBI) | 10810 |
| RRID | AB_2882822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UPY6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WASF3, also named WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with movement, invasion, and metastasis in different cancers such as breast cancer, gastric cancer, and prostate cancer. It has been shown that WASF3 levels are elevated in advanced-stage cancers compared to lower-grade tumors and normal tissue. The 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa proteins similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WASF3 antibody 67620-1-Ig | Download protocol |
| IHC protocol for WASF3 antibody 67620-1-Ig | Download protocol |
| IF protocol for WASF3 antibody 67620-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |