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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDFY1 Polyclonal antibody (13960-1-AP)

    WB analysis using 13960-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 13960-1-AP (WDFY1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using WDFY1 Polyclonal antibody (13960-1-AP)

    WB analysis using 13960-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13960-1-AP (WDFY1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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WDFY1 Polyclonal antibody

WDFY1 Polyclonal Antibody for WB, ELISA
Cat No. 13960-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

WD repeat and FYVE domain-containing protein 1, Phosphoinositide-binding protein 1, KIAA1435, FYVE domain-containing protein localized to endosomes 1, FENS-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, mouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13960-1-AP targets WDFY1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDFY1 fusion protein Ag4877 相同性解析による交差性が予測される生物種
Full Name WD repeat and FYVE domain containing 1
Calculated molecular weight 410 aa, 46 kDa
Observed molecular weight43-46 kDa
GenBank accession numberBC040525
Gene Symbol WDFY1
Gene ID (NCBI) 57590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WD repeating domain FYVE1 (WDFY1) belongs to the WD40 protein family, which plays key roles in diverse cellular processes, including signal transduction, gene transcription, vesicle fusion, and spermatogenesis(PMID: 32867693; PMID: 10322433). WDFY1 is highly expressed in mouse testis and is dispensable for mouse spermatogenesis and male fertility (PMID: 35121371). The bands of 24 kDa and 19 kDa may be potential isoforms of WDFY1.

Protocols

Product Specific Protocols
WB protocol for WDFY1 antibody 13960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols