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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human tonsillitis using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human tonsillitis using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of mouse brain using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of mouse brain using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human prostate cancer using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human prostate cancer using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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WDFY4 Polyclonal antibody

WDFY4 Polyclonal Antibody for IHC, ELISA
Cat No. 17558-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

C10orf64, KIAA1607, WDFY family member 4, WDFY4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17558-1-AP targets WDFY4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDFY4 fusion protein Ag11720 相同性解析による交差性が予測される生物種
Full Name WDFY family member 4
Calculated molecular weight 354 kDa
Observed molecular weight ~70 kDa
GenBank accession numberBC032420
Gene Symbol WDFY4
Gene ID (NCBI) 57705
RRIDAB_2288447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZS81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDFY4, also named as C10orf64 and KIAA1607, is a huge protein with unknown functions. WDFY4 is expressed predominantly in immune tissues such as lymph nodes, the spleen, the thymus and the tonsils. According to recent genome-wide association studies (GWAS), WDFY4 was introduced as novel predisposing genes for SLE (Systemic Lupus Erythematosus) which is a heterogeneous complex relapsing-remitting autoimmune disease. This antibody detects the C-terminal of WDFY4. The gene WDFY4 has some isoforms, 354 kDa, 73 kDa, 115 kDa, 353 kDa, 325 kDa( from UNIPROT) and 1992aa, 222 kDa; 3224aa, 358kDa; 624aa, 69 kDa; 1270aa, 143 kDa; 1887aa, 211 kDa (From NCBI).

Protocols

Product Specific Protocols
IHC protocol for WDFY4 antibody 17558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols