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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WDR21C Recombinant antibody, PBS Only (Detector)

WDR21C Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240402H3

Cat no : 83802-4-PBS

Synonyms

WD repeat-containing protein 21C, WD repeat domain 21C, DDB1- and CUL4-associated factor 4-like protein 2, DCAF4L2, 240402H3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using WDR21C Recombinant antibody (83802-4-RR)

    WB analysis using 83802-4-RR (same clone as 83802-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83802-4-RR (WDR21C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83802-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00738-2

    Cytometric bead array standard curve of MP00738-2

    Cytometric bead array standard curve of MP00738-2, WDR21C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83802-1-PBS. Detection antibody: 83802-4-PBS. Standard: Ag30748. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83802-4-RR

    Affinity and Kinetic Characterization of 83802-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83802-4-RR against Human WDR21C were performed. The affinity constant is 0.479 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83802-4-PBS targets WDR21C as part of a matched antibody pair:

MP00738-2: 83802-1-PBS capture and 83802-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WDR21C fusion protein Ag30748 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 21C
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC111778
Gene symbol WDR21C
Gene ID (NCBI) 138009
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.