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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WDR32 Polyclonal antibody

WDR32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20483-1-AP

Synonyms

DCAF10, WD repeat domain 32, WDR32

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using WDR32 Polyclonal antibody (20483-1-AP)

    WB analysis of HeLa using 20483-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using WDR32 Polyclonal antibody (20483-1-AP)

    WB analysis of HepG2 using 20483-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using WDR32 Polyclonal antibody (20483-1-AP)

    IP experiment of HepG2 using 20483-1-AP

    IP result of anti-WDR32 (IP:20483-1-AP, 4ug; Detection:20483-1-AP 1:300) with HepG2 cells lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR32 Polyclonal antibody (20483-1-AP)

    IHC staining of human testis using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR32 Polyclonal antibody (20483-1-AP)

    IHC staining of human testis using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using WDR32 Polyclonal antibody (20483-1-AP)

    IHC staining of human kidney using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using WDR32 Polyclonal antibody (20483-1-AP)

    IHC staining of human kidney using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WDR32 Polyclonal antibody (20483-1-AP)

    IF Staining of HepG2 using 20483-1-AP

    Immunofluorescent analysis of HepG2 cells using 20483-1-AP (WDR32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20483-1-AP is WDR32 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR32 fusion protein Ag14360 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 32
Calculated molecular weight 559 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC003520
Gene symbol WDR32
Gene ID (NCBI) 79269
RRIDAB_10695183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech's WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.

Protocols

Product Specific Protocols
WB protocol for WDR32 antibody 20483-1-APDownload protocol
IHC protocol for WDR32 antibody 20483-1-APDownload protocol
IF protocol for WDR32 antibody 20483-1-APDownload protocol
IP protocol for WDR32 antibody 20483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols