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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using WDR35 Polyclonal antibody (26701-1-AP)

    IF Staining of MDCK using 26701-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using WDR35 antibody (26701-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using WDR35 Polyclonal antibody (26701-1-AP)

    IF Staining of hTERT-RPE1 using 26701-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR35 antibody (26701-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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WDR35 Polyclonal antibody

WDR35 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 26701-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

IF/ICC, ELISA

Synonyms

IFT121, KIAA1336, WD repeat domain 35, WDR35

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26701-1-AP targets WDR35 in IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR35 fusion protein Ag24965 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 35
Calculated molecular weight 1170 aa, 132 kDa
GenBank accession numberBC036659
Gene Symbol WDR35
Gene ID (NCBI) 57539
RRIDAB_3085893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2L0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR35, a component of the IFT complex A (IFT-A), is required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). It is involved in ciliogenesis and ciliary protein trafficking (PMID:21473986, PMID:28400947, PMID:29220510). It may promote CASP3 activation and TNF-stimulated apoptosis (PMID: 20193664).

Protocols

Product Specific Protocols
IF protocol for WDR35 antibody 26701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols