- Featured Product
- KD/KO Validated
WDR45/WIPI4 Polyclonal antibody
WDR45/WIPI4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 19194-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 11 publications below |
| IP | See 1 publications below |
Product Information
19194-1-AP targets WDR45/WIPI4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WDR45/WIPI4 fusion protein Ag6741 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 45 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC000464 |
| Gene symbol | WDR45 |
| Gene ID (NCBI) | 11152 |
| RRID | AB_2215404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR45/WIPI4 antibody 19194-1-AP | Download protocol |
| IHC protocol for WDR45/WIPI4 antibody 19194-1-AP | Download protocol |
| IF protocol for WDR45/WIPI4 antibody 19194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Sci Adv Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.
| ||
Int J Mol Sci Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.
| ||
J Neurochem A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation
| ||
Am J Med Genet A Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration. | ||
Eur J Med Genet Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants. |
