WDR5 Polyclonal antibody

WDR5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15544-1-AP

Synonyms

BIG 3, BIG3, BMP2 induced 3 kb gene protein, SWD3, WD repeat containing protein 5, WD repeat domain 5, WDR5


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Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15544-1-AP targets WDR5 in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR5 fusion protein Ag7900 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 5
Calculated molecular weight 37 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC001635
Gene symbol WDR5
Gene ID (NCBI) 11091
RRIDAB_2257220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WDR5 antibody targets the WD repeat protein domain 5 (WDR5), a member of the WD40 repeat protein family in humans. The WDR5 protein is a core member of the mammalian trithorax complex and, like the rest of the WD40 repeat protein family, is primarily involved in diverse cellular processes. WDR5 is identified as a methyl Lys-4 H3-specific-binding protein, however it is known to interact with the Mixed Lineage Leukemia (MLL) protein at the same site for histone H3 tri-methylation(PMID:18840606). Recently, the WDR5 protein has been shown to regulate embryonic stem cell self-renewal, and an increased expression of WDR5 has been found to efficiently induce the formation of pluripotent stem cells (PMID:21477851). WDR5 is also shown to accelerate osteoblast and chondrocyte differentiation(PMID:16730692 ).

Protocols

Product Specific Protocols
WB protocol for WDR5 antibody 15544-1-APDownload protocol
IHC protocol for WDR5 antibody 15544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics

Authors - Mai Abdel Mouti
humanChIP

Sci Adv

A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.

Authors - Austin G Holmes
humanWB,IF

Proc Natl Acad Sci U S A

Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.

Authors - Ma Pengpeng P
humanWB

Pharmacol Res

HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5

Authors - Fu-Chun Huo
mouseWB

Nat Aging

Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging

Authors - Meiyang Li
WB

Mol Med

Glucagon-like peptide-1 receptor agonist exendin 4 ameliorates diabetes-associated vascular calcification by regulating mitophagy through the AMPK signaling pathway

Authors - Kui Chen