WDR61 Polyclonal antibody
WDR61 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 22536-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IP | See 1 publications below |
Product Information
22536-1-AP targets WDR61 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WDR61 fusion protein Ag18443 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 61 |
| Calculated molecular weight | 305 aa, 34 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC010080 |
| Gene symbol | WDR61 |
| Gene ID (NCBI) | 80349 |
| RRID | AB_11232419 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency (refer to UniProt). Catalog#22536-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR61. The MW of this protein is 33 kDa, and this antibody specially recognises the 33 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR61 antibody 22536-1-AP | Download protocol |
| IHC protocol for WDR61 antibody 22536-1-AP | Download protocol |
| IF protocol for WDR61 antibody 22536-1-AP | Download protocol |
| IP protocol for WDR61 antibody 22536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer | ||
Mol Cancer Ther Targeting the synthetic lethal relationship between FOCAD and TUT7 represents a potential therapeutic opportunity for TUT4/7 small molecule inhibitors in cancer | ||
Cell Death Dis UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer. | ||
FEBS J WDR61 ablation triggers R-loop accumulation and suppresses breast cancer progression |
