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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WDR62 Polyclonal antibody

WDR62 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23404-1-AP

Synonyms

WD repeat-containing protein 62, WD repeat domain 62, C19orf14

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293T cells using WDR62 Polyclonal antibody (23404-1-AP)

    IP experiment of HEK-293T using 23404-1-AP

    IP result of anti-WDR62 (IP:23404-1-AP, 4ug; Detection:23404-1-AP 1:500) with HEK-293T cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR62 Polyclonal antibody (23404-1-AP)

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR62 Polyclonal antibody (23404-1-AP)

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using WDR62 Polyclonal antibody (23404-1-AP)

    IF Staining of hTERT-RPE1 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using WDR62 Polyclonal antibody (23404-1-AP)

    IF Staining of A549 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IP detected inHEK-293T cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, A549 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23404-1-AP targets WDR62 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR62 fusion protein Ag19865 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 62
Calculated molecular weight 1518 aa, 166 kDa
GenBank accession numberBC017261
Gene symbol WDR62
Gene ID (NCBI) 284403
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571).

Protocols

Product Specific Protocols
IHC protocol for WDR62 antibody 23404-1-APDownload protocol
IF protocol for WDR62 antibody 23404-1-APDownload protocol
IP protocol for WDR62 antibody 23404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols