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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WDR74 Polyclonal antibody

WDR74 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20631-1-AP

Synonyms

NOP seven associated protein 1, NSA1, WD repeat domain 74, WDR74

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of HepG2 using 20631-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of MCF-7 using 20631-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of HepG2 using 20631-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using WDR74 Polyclonal antibody (20631-1-AP)

    WB analysis of K-562 using 20631-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20631-1-AP is WDR74 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR74 fusion protein Ag14617 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 74
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC006351
Gene symbol WDR74
Gene ID (NCBI) 54663
RRIDAB_2878713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WDR74 antibody 20631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols