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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WDR77 Polyclonal antibody

WDR77 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10115-1-AP

Synonyms

WDR 77, WD45, WD repeat-containing protein 77, Nbla10071, Methylosome protein WDR77

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR77 Polyclonal antibody (10115-1-AP)

    WB analysis using 10115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10115-1-AP (WDR77 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using WDR77 Polyclonal antibody (10115-1-AP)

    IHC staining of human stomach using 10115-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10115-1-AP (WDR77 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10115-1-AP targets WDR77 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR77 fusion protein Ag0147 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 77
Calculated molecular weight 50 kDa
Observed molecular weight40 kDa
GenBank accession numberBC001679
Gene symbol WDR77
Gene ID (NCBI) 79084
RRIDAB_2215725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR77, other named p44, MEP-50, is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. Normally, WDR77 locates in the nucleus, such as in prostate epithelial cells. However, it localized to the cytoplasm in prostatic intraepithelial neoplasia and prostate cancer cells. Nuclear WDR77 mediates the growth inhibition and differentiation. WDR may involve in the biogenesis of small nuclear ribonucleoprotein particles.

Protocols

Product Specific Protocols
WB protocol for WDR77 antibody 10115-1-APDownload protocol
IHC protocol for WDR77 antibody 10115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression

Authors - Yan Sun
View Article