Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10115-1-AP targets WDR77 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WDR77 fusion protein Ag0147 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 77 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC001679 |
| Gene Symbol | WDR77 |
| Gene ID (NCBI) | 79084 |
| RRID | AB_2215725 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WDR77, other named p44, MEP-50, is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. Normally, WDR77 locates in the nucleus, such as in prostate epithelial cells. However, it localized to the cytoplasm in prostatic intraepithelial neoplasia and prostate cancer cells. Nuclear WDR77 mediates the growth inhibition and differentiation. WDR may involve in the biogenesis of small nuclear ribonucleoprotein particles.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR77 antibody 10115-1-AP | Download protocol |
| IHC protocol for WDR77 antibody 10115-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression |