Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, SH-SY5Y cells |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
24874-1-AP targets WDR81 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WDR81 fusion protein Ag21666 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 81 |
| Calculated molecular weight | 1941 aa, 212 kDa |
| Observed molecular weight | 74-80 kDa, 250 kDa |
| GenBank accession number | BC114568 |
| Gene Symbol | WDR81 |
| Gene ID (NCBI) | 124997 |
| RRID | AB_2879771 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q562E7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WD repeat-containing protein 81 (WDR81) is a 1941 amino acid protein, which contains one BEACH domain and five WD repeats. WDR81 is widely expressed in the brain. WDR81 has an association with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 2. WDR81 exists as six isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR81 antibody 24874-1-AP | Download protocol |
| IHC protocol for WDR81 antibody 24874-1-AP | Download protocol |
| IF protocol for WDR81 antibody 24874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Psychiatry WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.
| ||
Neurogenetics Fetal brain arrest broadens the spectrum of WDR81-related developmental brain malformations. | ||
Elife PDGFRα signaling regulates Srsf3 transcript binding to affect PI3K signaling and endosomal trafficking |