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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:400 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using WFS1 Polyclonal antibody (26995-1-AP)

    IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WFS1 Polyclonal antibody (26995-1-AP)

    IF Staining of HepG2 using 26995-1-AP (same clone as 26995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

WFS1 Polyclonal antibody, PBS Only

WFS1 Polyclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 26995-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26995-1-PBS targets WFS1 in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WFS1 fusion protein Ag25724 相同性解析による交差性が予測される生物種
Full Name Wolfram syndrome 1 (wolframin)
Calculated molecular weight 890 aa, 100 kDa
GenBank accession numberBC030130
Gene Symbol WFS1
Gene ID (NCBI) 7466
RRIDAB_2880717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76024
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.