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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WFS1 Monoclonal antibody, PBS Only

WFS1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C2B5

Cat no : 67291-1-PBS

Synonyms

FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis using 67291-1-Ig (same clone as 67291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A172 cells using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis of A172 using 67291-1-Ig (same clone as 67291-1-PBS)

    A172 cells were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis using 67291-1-Ig (same clone as 67291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis using 67291-1-Ig (same clone as 67291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67291-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67291-1-PBS targets WFS1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WFS1 fusion protein Ag25735 相同性解析による交差性が予測される生物種
Full Name Wolfram syndrome 1 (wolframin)
Calculated molecular weight 890 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030130
Gene symbol WFS1
Gene ID (NCBI) 7466
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.