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Validation Data Gallery

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  • Western Blot (WB) analysis of HCT 116 cells using WHSC1 Polyclonal antibody (22722-1-AP)

    WB analysis of HCT 116 using 22722-1-AP (same clone as 22722-1-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 22722-1-AP (WHSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22722-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using WHSC1 Polyclonal antibody (22722-1-AP)

    IP experiment of HeLa using 22722-1-AP (same clone as 22722-1-PBS)

    IP result of anti-WHSC1 (IP:22722-1-AP, 3ug; Detection:22722-1-AP 1:300) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 22722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WHSC1 Polyclonal antibody (22722-1-AP)

    IHC staining of human breast cancer using 22722-1-AP (same clone as 22722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22722-1-AP (WHSC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WHSC1 Polyclonal antibody (22722-1-AP)

    IHC staining of human breast cancer using 22722-1-AP (same clone as 22722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22722-1-AP (WHSC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WHSC1 Polyclonal antibody (22722-1-AP)

    IHC staining of human breast cancer using 22722-1-AP (same clone as 22722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22722-1-AP (WHSC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22722-1-PBS in a different storage buffer formulation.

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WHSC1 Polyclonal antibody, PBS Only

WHSC1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 22722-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22722-1-PBS targets WHSC1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WHSC1 fusion protein Ag18611 相同性解析による交差性が予測される生物種
Full Name Wolf-Hirschhorn syndrome candidate 1
Calculated molecular weight 162 kDa
Observed molecular weight 70 kDa, 150 kDa
GenBank accession numberBC152412
Gene Symbol WHSC1
Gene ID (NCBI) 7468
RRIDAB_2879156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO96028
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

WHSC1 (also known as MMSET or NSD2) is a SET domain containing histone lysine methyltransferase that can di- and trimethylate histone H3 at lysine 36 (H3K36). WHSC1 mRNA is highly expressed in embryonic tissues, especially those with high degrees of proliferation, whereas in adult tissues,the mRNA is primarily expressed in thymus and testis. WHSC1 is implicated in cellular adhesion, clonogenic growth and tumorigenicity. Its overexpression has been found in various cancers. It undergoes alternative splicing resulting in different protein isoforms. Recently WHSC1 has been identified as a critical epigenetic regulator for Tfh differentiation.