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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse colon tissue using WIBG Polyclonal antibody (14658-1-AP)

    IHC staining of mouse colon using 14658-1-AP (same clone as 14658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14658-1-AP (WIBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14658-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using WIBG Polyclonal antibody (14658-1-AP)

    IF Staining of U2OS using 14658-1-AP (same clone as 14658-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using WIBG antibody (14658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14658-1-PBS in a different storage buffer formulation.

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WIBG Polyclonal antibody, PBS Only

WIBG Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 14658-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14658-1-PBS targets WIBG in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIBG fusion protein Ag6276 相同性解析による交差性が予測される生物種
Full Name within bgcn homolog (Drosophila)
Calculated molecular weight 23 kDa
GenBank accession numberBC006135
Gene Symbol WIBG
Gene ID (NCBI) 84305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRP8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).