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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WIPF1 Polyclonal antibody, PBS Only

WIPF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22047-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WIPF1 Polyclonal antibody (22047-1-AP)

    WB analysis using 22047-1-AP (same clone as 22047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22047-1-AP (WIPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using WIPF1 Polyclonal antibody (22047-1-AP)

    IHC staining of human stomach cancer using 22047-1-AP (same clone as 22047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using WIPF1 Polyclonal antibody (22047-1-AP)

    IHC staining of human stomach cancer using 22047-1-AP (same clone as 22047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22047-1-PBS targets WIPF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIPF1 fusion protein Ag16975 相同性解析による交差性が予測される生物種
Full Name WAS/WASL interacting protein family, member 1
Calculated molecular weight403aa,40 kDa; 503aa,51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC110288
Gene symbol WIPF1
Gene ID (NCBI) 7456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429).