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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WIPF1 Polyclonal antibody (22047-1-AP)

    WB analysis using 22047-1-AP (same clone as 22047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22047-1-AP (WIPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using WIPF1 Polyclonal antibody (22047-1-AP)

    IHC staining of human stomach cancer using 22047-1-AP (same clone as 22047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using WIPF1 Polyclonal antibody (22047-1-AP)

    IHC staining of human stomach cancer using 22047-1-AP (same clone as 22047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

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WIPF1 Polyclonal antibody, PBS Only

WIPF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22047-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22047-1-PBS targets WIPF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIPF1 fusion protein Ag16975 相同性解析による交差性が予測される生物種
Full Name WAS/WASL interacting protein family, member 1
Calculated molecular weight403aa,40 kDa; 503aa,51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC110288
Gene Symbol WIPF1
Gene ID (NCBI) 7456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43516
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429).