Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, A549 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68149-1-Ig targets WNK1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | WNK1 fusion protein Ag28044 相同性解析による交差性が予測される生物種 |
| Full Name | WNK lysine deficient protein kinase 1 |
| Calculated molecular weight | 251 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | BC021121 |
| Gene Symbol | WNK1 |
| Gene ID (NCBI) | 65125 |
| RRID | AB_2923673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H4A3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WNK1 antibody 68149-1-Ig | Download protocol |
| FC protocol for WNK1 antibody 68149-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |