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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human heart tissue using WNK2 Polyclonal antibody (22297-1-AP)

    IHC staining of human heart using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using WNK2 Polyclonal antibody (22297-1-AP)

    IHC staining of human heart using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WNK2 Polyclonal antibody (22297-1-AP)

    IHC staining of human lung cancer using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WNK2 Polyclonal antibody (22297-1-AP)

    IHC staining of human lung cancer using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WNK2 Polyclonal antibody (22297-1-AP)

    IF Staining of HepG2 using 22297-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22297-1-AP (WNK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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WNK2 Polyclonal antibody

WNK2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 22297-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22297-1-AP targets WNK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name WNK lysine deficient protein kinase 2
Calculated molecular weight 243 kDa
GenBank accession numberNM_006648
Gene Symbol WNK2
Gene ID (NCBI) 65268
RRIDAB_2935457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3S1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for WNK2 antibody 22297-1-APDownload protocol
IF protocol for WNK2 antibody 22297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols