Validation Data Gallery

Filter: AllWBIF/ICCFC (Intra)

WB analysis using 27935-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 27935-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 27935-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of NIH/3T3 using 27935-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 27935-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT1 antibody (27935-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of NIH/3T3 using 27935-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
FC experiment of HeLa using 27935-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human WNT1 (27935-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

WNT1 Polyclonal antibody

WNT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 27935-1-AP

Publications(46)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, Proto oncogene Wnt 1, Proto oncogene Int 1 homolog, INT1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (46)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	3T3-L1 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells
Positive IF/ICC detected in	NIH/3T3 cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 46 publications below
IHC	See 6 publications below
IF	See 1 publications below

Product Information

27935-1-AP targets WNT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	WNT1 fusion protein Ag27380 相同性解析による交差性が予測される生物種
Full Name	wingless-type MMTV integration site family, member 1
Observed molecular weight	41 kDa
GenBank accession number	BC074799
Gene Symbol	WNT1
Gene ID (NCBI)	7471
RRID	AB_2881013
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04628
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for WNT1 antibody 27935-1-AP	Download protocol
IF protocol for WNT1 antibody 27935-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Bioengineered MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) Authors - Guang-Lin Song View Article
human	WB	Neural Regen Res Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke Authors - Jianfeng Wang View Article
human	WB	Aging (Albany NY) Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway. Authors - Liansheng Sha View Article
mouse	WB	J Ethnopharmacol Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice Authors - Yin OuYang View Article
mouse	WB	Microb Biotechnol Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice. Authors - Xiaoxiao Zhao View Article
human	WB,IHC	Front Oncol Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6. Authors - Hanqing Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27935-1-AP

WB analysis using 27935-1-AP

WB analysis using 27935-1-AP

IF Staining of NIH/3T3 using 27935-1-AP

IF Staining of HeLa using 27935-1-AP

IF Staining of NIH/3T3 using 27935-1-AP

FC experiment of HeLa using 27935-1-AP

WNT1 Polyclonal antibody

WNT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)

Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice

Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.

Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.