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- KD/KO Validated
WNT1 Polyclonal antibody
WNT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 27935-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | 3T3-L1 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 42 publications below |
| IHC | See 6 publications below |
| IF | See 1 publications below |
Product Information
27935-1-AP targets WNT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WNT1 fusion protein Ag27380 相同性解析による交差性が予測される生物種 |
| Full Name | wingless-type MMTV integration site family, member 1 |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC074799 |
| Gene symbol | WNT1 |
| Gene ID (NCBI) | 7471 |
| RRID | AB_2881013 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WNT1 antibody 27935-1-AP | Download protocol |
| IF protocol for WNT1 antibody 27935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioengineered MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) | ||
Neural Regen Res Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke | ||
Aging (Albany NY) Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway. | ||
Microb Biotechnol Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice. | ||
Front Oncol Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6. | ||
Transplantation Agmatine Attenuates Liver Ischemia Reperfusion Injury by Activating Wnt/β-catenin Signaling in Mice. |
