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  • Featured Product
  • KD/KO Validated

WNT10B Monoclonal antibody, PBS Only

WNT10B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G11E4

Cat no : 67210-1-PBS

Synonyms

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT10B Monoclonal antibody (67210-1-Ig)

    WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67210-1-Ig (WNT10B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse skeletal muscle using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of human heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of human heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse skeletal muscle using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using WNT10B Monoclonal antibody (67210-1-Ig)

    IHC staining of mouse heart using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WNT10B Monoclonal antibody (67210-1-Ig)

    IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67210-1-Ig (WNT10B antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67210-1-PBS targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WNT10B fusion protein Ag17114 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 10B
Calculated molecular weight 389 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC096353
Gene symbol WNT10B
Gene ID (NCBI) 7480
RRIDAB_2882503
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.