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KD/KO Validated

WNT2 Polyclonal antibody

Name: WNT2 Polyclonal antibody
Brand: Proteintech
SKU: 27214-1-AP

WNT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27214-1-AP

Synonyms

IRP, Int-1-related protein, Int-1-like protein 1, INT1L1, Int 1 related protein

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 27214-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 27214-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 27214-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of rat brain using 27214-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27214-1-AP (WNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27214-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27214-1-AP (WNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 27214-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT2 antibody (27214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of Jurkat using 27214-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human WNT2 (27214-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 27214-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue, MCF-7 cells
Positive IHC detected in	rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

27214-1-AP targets WNT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	WNT2 fusion protein Ag25845 相同性解析による交差性が予測される生物種
Full Name	wingless-type MMTV integration site family member 2
Calculated molecular weight	40 kDa
Observed molecular weight	40 kDa, 34 kDa
GenBank accession number	BC029854
Gene symbol	WNT2
Gene ID (NCBI)	7472
RRID	AB_2880804
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

Protocols

Product Specific Protocols
WB protocol for WNT2 antibody 27214-1-AP	Download protocol
IHC protocol for WNT2 antibody 27214-1-AP	Download protocol
IF protocol for WNT2 antibody 27214-1-AP	Download protocol
FC protocol for WNT2 antibody 27214-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Clin Transl Med Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway. Authors - Xi Dong View Article
human	WB	Aging (Albany NY) LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p. Authors - Liting Jin View Article
mouse	WB	FASEB J Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2. Authors - Yunzhen Ye View Article
human	WB	Phytother Res Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway. Authors - Mingming Zhu View Article
mouse	WB	Brain Res Bull Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring. Authors - Qian Wu View Article
human	WB	Cancer Med Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling. Authors - Xia Liu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNT2 Polyclonal antibody

WNT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 27214-1-AP

WB analysis using 27214-1-AP

WB analysis using 27214-1-AP

IHC staining of rat brain using 27214-1-AP

IHC staining of mouse brain using 27214-1-AP

IF Staining of MCF-7 using 27214-1-AP

FC experiment of Jurkat using 27214-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.

Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2.

Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.

Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.

Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.