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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNT3A Recombinant antibody, PBS Only (Capture)

WNT3A Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241993D6

Cat no : 84577-5-PBS

Synonyms

Protein Wnt-3a, Protein Wnt 3a, 241993D6

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01423-3

    Sandwich ELISA standard curve of MP01423-3

    Sandwich ELISA standard curve of MP01423-3, Human WNT3A Recombinant Matched Antibody Pair - PBS only. 84577-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25051. 84577-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01423-3

    Sample test of MP01423-3

    The mean WNT3A concentration was determined to be 999.1 pg/mL in MDA-MB-231 cell extract based on a 1.2 mg/mL extract load and 2,063.4 pg/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

  • Sample test of MP01423-3

    Sample test of MP01423-3

    Serum of six individual healthy human donors was measured. The WNT3A concentration of detected samples was determined to be 2,012.8 pg/mL with a range of 101.5-6,680.8 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84577-5-PBS targets WNT3A as part of a matched antibody pair:

MP01423-3: 84577-5-PBS capture and 84577-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WNT3A fusion protein Ag25051 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 3A
Calculated molecular weight 352 aa, 39 kDa
GenBank accession numberBC103921
Gene symbol WNT3A
Gene ID (NCBI) 89780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.