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WNT5A/B Polyclonal antibody

WNT5A/B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55184-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, IP, RIP, ELISA

WNT5A, WNT5B, WNT5A B, Protein Wnt-5a, Protein Wnt 5a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse heart tissue, HUVEC cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue, human tonsillitis tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55184-1-AP targets WNT5A/B in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 5A
Calculated molecular weight 42 kDa
Observed molecular weight 38 kDa
GenBank accession numberNM_003392
Gene Symbol WNT5A
Gene ID (NCBI) 7474
RRIDAB_2881285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.

Protocols

Product Specific Protocols
WB protocol for WNT5A/B antibody 55184-1-APDownload protocol
IHC protocol for WNT5A/B antibody 55184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanIHC

Genome Biol

Integrated genomic and transcriptomic analysis reveals unique characteristics of hepatic metastases and pro-metastatic role of complement C1q in pancreatic ductal adenocarcinoma.

Authors - Jianyu Yang
humanWB

Acta Biomater

Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.

Authors - Hong Cao

Biomaterials

Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.

Authors - Yonglin Yu
humanWB

Nanoscale

An assembly-inducing PDC enabling the efficient nuclear delivery of nucleic acid for cancer stem-like cell suppression

Authors - Dongyuan Wang
mouseWB,IF,RIP

Arch Med Res

RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway

Authors - Zhao Yan
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