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  • Featured Product
  • KD/KO Validated

WNT5A/B Polyclonal antibody, PBS Only

WNT5A/B Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55184-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT5A/B Polyclonal antibody (55184-1-AP)

    WB analysis using 55184-1-AP (same clone as 55184-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WNT5A/B Polyclonal antibody (55184-1-AP)

    WB analysis using 55184-1-AP (same clone as 55184-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using WNT5A/B Polyclonal antibody (55184-1-AP)

    WB analysis of PC-3 using 55184-1-AP (same clone as 55184-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using WNT5A/B Polyclonal antibody (55184-1-AP)

    IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55184-1-PBS targets WNT5A/B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 5A
Calculated molecular weight 42 kDa
Observed molecular weight 38 kDa
GenBank accession numberNM_003392
Gene symbol WNT5A
Gene ID (NCBI) 7474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.